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http://hdl.handle.net/10119/10609
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| Title: | Labelless impedance immunosensor based on polypyrrole-pyrolecarboxylic acid copolymer for hCG detection |
| Authors: | Truong, Lien T.N.
Chikae, Miyuki
Ukita, Yoshiaki
Takamura, Yuzuru |
| Keywords: | human Chorionic Gonadotropin (hCG)
label-free impedimetric immunosensor
conducting polymer
polypyrrole
electrochemical impedance spectroscopy (EIS) |
| Issue Date: | 2011-08-16 |
| Publisher: | Elsevier |
| Magazine name: | Talanta |
| Volume: | 85 |
| Number: | 5 |
| Start page: | 2576 |
| End page: | 2580 |
| DOI: | 10.1016/j.talanta.2011.08.018 |
| Abstract: | In this work, a sensitive label-free impedimetric hCG-immunosensor was constructed by using a commercial screen-printing carbon ink electrode (namely Disposable Electrochemical Printed chip) as the basis. The carbon ink electrode of DEP chip is modified first by deposition of polypyrrole–pyrole-2-carborxylic acid copolymer and thence hCG antibody immobilization via the COOH groups of pyrrole-2-carboxylic acid, which can serve as linker for covalent biomolecular immobilization. The experimental results exposed that the designed immunosensor is more sensitive than other previously reported immunosensors, in the case of detection limit and linear range for antigen detection. With optimal fabrication parameters, the detection limit for α-hCG was 2.3 pg/mL in 10mM phosphate buffer saline (PBS) solution containing 1% Bovine Serum Albumine (BSA). Moreover, the use of inexpensive DEP chip as a basis for these immunosensors will allow for simple instrumentation, disposable and portable at low cost. This work also demonstrates a new approach to develop a sensitive and label-free impedimetric immunosensor based on screen-printed electrode for applications in clinical diagnosis. |
| Rights: | NOTICE: This is the author's version of a work accepted for publication by Elsevier. Lien T.N. Truong, Miyuki Chikae, Yoshiaki Ukita and Yuzuru Takamura, Talanta, 85(5), 2011, 2576-2580, http://dx.doi.org/10.1016/j.talanta.2011.08.018 |
| URI: | http://hdl.handle.net/10119/10609 |
| Material Type: | author |
| Appears in Collections: | c10-1. 雑誌掲載論文 (Journal Articles)
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