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c10-1. 雑誌掲載論文 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/4933
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| タイトル: | Preparation and characterization of biodegradable nanoparticles based on poly(γ-glutamic acid) with L-phenylalanine as a protein carrier |
| 著者: | Akagi, Takami
Kaneko, Tatsuo
Kida, Toshiyuki
Akashi, Mitsuru |
| キーワード: | Poly (γ-glutamic acid)
Biodegradation
Nanoparticles
Amphiphilic
Encapsulation |
| 発行日: | 2005-11-28 |
| 出版者: | Elsevier |
| 誌名: | Journal of Controlled Release |
| 巻: | 108 |
| 号: | 2-3 |
| 開始ページ: | 226 |
| 終了ページ: | 236 |
| DOI: | 10.1016/j.jconrel.2005.08.003 |
| 抄録: | The objective of the present study was to prepare nanoparticles composed of poly (γ-glutamic acid) (γ-PGA) and L-phenylalanine ethylester (L-PAE) in order to evaluate the possibility of using these nanoparticles as protein carriers. Novel amphiphilic graft copolymers composed of γ-PGA as the hydrophilic backbone and L-PAE as the hydrophobic segment were successfully synthesized by grafting L-PAE to γ-PGA using water-soluble carbodiimide (WSC). Due to their amphiphilic properties, the γ-PGA-graft-L-PAE copolymers were able to form nanoparticles. The size of the γ-PGA nanoparticles was measured by photon correlation spectroscopy (PCS), and showed a narrow monodispersed size distribution with a mean diameter ranging from 150 to 200 nm. The solvents selected to prepare the γ-PGA nanoparticles by a precipitation and dialysis method affected the particle size distribution. To evaluate the feasibility of vehicles for these proteins, we prepared protein-loaded γ-PGA nanoparticles by surface immobilization and encapsulation methods. Ovalbumin (OVA) was used as a model protein, and was immobilized onto the γ-PGA nanoparticles or encapsulated into the inner core of these nanoparticles. Moreover, these OVA-encapsulated γ-PGA nanoparticles could be preserved by freeze-drying process. The results of cytotoxicity tests showed that the γ-PGA and γ-PGA nanoparticles did not cause any relevant cell damage. It is expected that biodegradable γ-PGA nanoparticles can immobilize proteins, peptides, plasmid DNA and drugs onto their surfaces and/or into the nanoparticles. These nanoparticles are potentially useful in pharmaceutical and biomedical applications. |
| Rights: | NOTICE: This is the author's version of a work accepted for publication by Elsevier. Takami Akagi, Tatsuo Kaneko, Toshiyuki Kida, and Mitsuru Akashi, Journal of Controlled Release, 108(2-3), 2005, 226-236, http://dx.doi.org/10.1016/j.jconrel.2005.08.003 |
| URI: | http://hdl.handle.net/10119/4933 |
| 資料タイプ: | author |
| 出現コレクション: | c10-1. 雑誌掲載論文 (Journal Articles)
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