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このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/9074
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タイトル: | Mass spectrometric imaging of ginsenosides localization in Panax ginseng root |
著者: | Taira, Shu Ikeda, Ryuzo Yokota, Naohiko Osaka, Issey Sakamoto, Manabu Kato, Mitsuro Sahashi, Yuko |
キーワード: | Mass spectrometry imaging panax ginseng ginsenoside localization |
発行日: | 2010 |
出版者: | World Scientific Publishing |
誌名: | The American Journal of Chinese Medicine |
巻: | 38 |
号: | 3 |
開始ページ: | 485 |
終了ページ: | 493 |
DOI: | 10.1142/S0192415X10008007 |
抄録: | We succeeded in performing mass spectrometric imaging (MSI) of the localization of ginsenosides (Rb_1, Rb_2 or R_c, and Rf) in cross-sections of the Panax ginseng root at a resolution of 100 μm using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Tandem mass spectrometry (MS/MS) of alkali metal-adducted ginsenoside ions revealed structural information of the corresponding saccharides and agricones. MALDI-MSI confirmed that localization of ginsenosides in the cortex and the periderm is higher than that in the medulla of a lateral root. In addition, it revealed that localization of ginsenosides in a root tip (diameter, 2.7 mm) is higher than that in the center of the root (diameter, 7.3 mm). A quantitative difference was detected between localizations of protopanaxadiol-type ginsenoside (Rb_1、 Rb_2, or R_c) and protopanaxatriol-type ginsenoside (Rf) in the root. This imaging approach is a promising technique for rapid evaluation and identification of medicinal saponins in plant tissues. |
Rights: | Electronic version of an article published as The American Journal of Chinese Medicine, 38(3), 2010, 485-493. DOI:10.1142/S0192415X10008007. Copyright World Scientific Publishing Company, http://dx.doi.org/10.1142/S0192415X10008007 |
URI: | http://hdl.handle.net/10119/9074 |
資料タイプ: | author |
出現コレクション: | c10-1. 雑誌掲載論文 (Journal Articles)
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15012-1.pdf | | 507Kb | Adobe PDF | 見る/開く |
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