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c10-1. 雑誌掲載論文 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/13700
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| タイトル: | Cryopreservation of a two-dimensional monolayer using a slow vitrification method with polyampholyte to inhibit ice crystal formation |
| 著者: | Matsumura, Kazuaki
Kawamoto, Keiko
Takeuchi, Masahiro
Yoshimura, Shigehiro
Tanaka, Daisuke
Hyon, Suong-Hyu |
| キーワード: | cryopreservation
ice crystallization
polyampholyte
tissue-engineered construct
vitrification |
| 発行日: | 2016-04-18 |
| 出版者: | American Chemical Society |
| 誌名: | ACS Biomaterials Science & Engineering |
| 巻: | 2 |
| 号: | 6 |
| 開始ページ: | 1023 |
| 終了ページ: | 1029 |
| DOI: | 10.1021/acsbiomaterials.6b00150 |
| 抄録: | Vitrification methods have been developed to improve the preservation of oocytes and embryos. However, successful vitrification and preservation typically requires very high cooling speeds. Here, we report a novel slow vitrification method for cryopreservation of two-dimensional (2D) cell constructs using a vitrification solution (VS) in PBS containing 6.5 M ethylene glycol, 0.5 M sucrose, and 10% w/w carboxylated poly-L-lysine (COOH-PLL), a novel polymeric cryoprotectant and stabilizing agent that is likely to inhibit ice crystallization. Stabilization of the glassy state and inhibition of devitrification was confirmed by thermal analysis using differential scanning calorimetry. The viability of cultured human mesenchymal stem cell (MSC) monolayers after freezing by our novel slow vitrification method at a rate of 4.9°C/min in VS with 10% COOH-PLL was significantly higher than that of cells frozen using our slow vitrification method in VS without COOH-PLL. Moreover, cells maintained the capacity for differentiation. We further confirmed that COOH-PLL improved the vitrification properties of the current vitrification system through inhibition of recrystallization properties. This novel, simple method for slow vitrification can be widely applicable for the preservation of tissue-engineered constructs and may facilitate the industrialization of regenerative medicine. |
| Rights: | Kazuaki Matsumura, Keiko Kawamoto, Masahiro Takeuchi, Shigehiro Yoshimura, Daisuke Tanaka, and Suong-Hyu Hyon, ACS Biomaterials Science & Engineering, 2016, 2(6), pp.1023-1029. This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in ACS Biomaterials Science & Engineering, copyright (c) American Chemical Society after peer review. To access the final edited and published work, see http://dx.doi.org/10.1021/acsbiomaterials.6b00150 |
| URI: | http://hdl.handle.net/10119/13700 |
| 資料タイプ: | author |
| 出現コレクション: | c10-1. 雑誌掲載論文 (Journal Articles)
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このアイテムのファイル:
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記述 |
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