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このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10119/4953
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| タイトル: | A globotriaosylceramide (Gb_3Cer) mimic peptide isolated from phage display library expressed strong neutralization to Shiga toxins |
| 著者: | Miura, Yoshiko
Sakaki, Akio
Kamihira, Masamichi
Iijima, Shinji
Kobayashi, Kazukiyo |
| キーワード: | Carbohydrate mimic peptide
Gb_3
Phage display library
Shiga toxin |
| 発行日: | 2006-06 |
| 出版者: | Elsevier |
| 誌名: | Biochimica et Biophysica Acta (BBA) : General Subjects |
| 巻: | 1760 |
| 号: | 6 |
| 開始ページ: | 883 |
| 終了ページ: | 889 |
| DOI: | 10.1016/j.bbagen.2006.03.018 |
| 抄録: | A Gb_3-trisaccharide mimic peptide was selected with biopanning from a phage display library against anti-Gb_3 antibody to neutralize Shiga toxins (Stxs). Biopanning was carried out on a microplate immobilized with a Fab fragment of anti-Gb_3 antibody and a subtraction procedure screening was applied to enhance specificity. The selected phage clones showed strong affinity to anti-Gb_3 antibody and to Stxs. Among these clones, a 9-mer sequence WHWTWLSEY was determined as the strongest Gb_3 mimic peptide and chemically synthesized. The peptide bound strongly to Stx-1 and Stx-2, though the binding was inhibited with Gb_3Cer. Surface plasmon resonance (SPR) and fluorescent spectroscopy determined that the affinity of the peptide to both Stxs was strong. Neutralization activity was confirmed by in vitro assay with HeLa cells. The Gb_3 mimic peptide potentially has great promise for use against Stxs. |
| Rights: | NOTICE: This is the author's version of a work accepted for publication by Elsevier. Yoshiko Miura, Akio Sakaki, Masamichi Kamihira, Shinji Iijima and Kazukiyo Kobayashi, Biochimica et Biophysica Acta (BBA) : General Subjects, 1760(6), 2006, 883-889, http://dx.doi.org/10.1016/j.bbagen.2006.03.018 |
| URI: | http://hdl.handle.net/10119/4953 |
| 資料タイプ: | author |
| 出現コレクション: | c10-1. 雑誌掲載論文 (Journal Articles)
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このアイテムのファイル:
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記述 |
サイズ | 形式 |
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| C6007.pdf | | 93Kb | Adobe PDF | 見る/開く |
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